RECOMBINANT LENTIVIRUS VECTOR TARGETING BOVINE LXRΑ MRNA AND ITS SILENCING EFFECTS ON BOVINE MUSCLE SATELLITE CELLS

Authors: Y. Liu, P. Lib, L. Zhao, Z. Zhao, Y. Li, M. Liu, X. Yang, L. Zan
Journal: Journal of Animal and Plant Sciences (JAPS)
ISSN: 1018-7081 (Print), 2309-8694 (Online)
Volume: 27  Issue: 6  Pages: 1847-1851
Year: 2017
DOI: NA
URL: https://doi.org/NA
Publisher: Pakistan Agricultural Scientists Forum

Abstract:
<p>The liver X receptor &alpha; (<em>LXR&alpha;)&nbsp;</em>is a member of the nuclear hormone receptor superfamily which could regulate the transcription of the genes involved in cholesterol transportation. In this study, Lentiviral vector containing<em>&nbsp;LXR&alpha;&nbsp;</em>shRNAs were constructed and transferred in bovine muscle satellite cells. The results indicated that&nbsp;<em>LXR&alpha;&nbsp;</em>down regulated&nbsp;<em>PPAR&alpha;</em>,&nbsp;<em>PPAR&gamma;</em>,&nbsp;<em>ABCA1</em>,&nbsp;<em>LPL</em>,&nbsp;<em>ApoE&nbsp;</em>genes after 48 h post-pLenti-03 viruses' infection. The silencing effects of bovine&nbsp;<em>LXR&alpha;&nbsp;</em>gene on related were significant. This work identifies bovine<em>&nbsp;LXR&alpha;&nbsp;</em>role in cholesterol metabolism.</p>

Keywords: bovine LXRα gene, lentivirus, vector construction, gene silencing