EFFECT OF COLCHICINE ON HUMORAL IMMUNITY AND IMMUNE ORGANS IN MICE

Authors: S. Khurram, A.Javeed1, M.Ashraf, J.Nazir, A.Ghafoor, G. Mustafa
Journal: Journal of Animal and Plant Sciences (JAPS)
ISSN: 1018-7081 (Print), 2309-8694 (Online)
Volume: 27  Issue: 4  Pages: 1142-1149
Year: 2017
DOI: NA
URL: https://doi.org/NA
Publisher: Pakistan Agricultural Scientists Forum

Abstract:
<p>This study was planned to investigate effect of colchicine on humoral immune responses and immune organs in mice. Humoral immune responses were evaluated by performing haemagglutination assay, mice lethality test and Jerne hemolytic plaque formation. Colchicine treated groups were administered 40&micro;g/kg, 80&micro;g/kg and 160&micro;g/kg.Data obtained was analyzed by ANOVA. There was significant(P &lt;0.0001)reduction in HA titer of colchicine treatedgroups. With the increasing dose, there was reduction in the HA titer. Colchicine enhanced the mortality after administration of bovine&nbsp;<em>Pasteurellamultocida</em>culture. In addition, there was significant (P&lt;0.0001) reduction in body weight and spleen weight difference of mice in colchicine treated groups. There were no histopathological changes observed in spleen and thymus at 40&micro;g/kg and 80&micro;g/kg doses of colchicine. At 160&micro;g/kg dose, increase in thickness of trabecular was seen due to edema in the spleen. This study has shown that humoral immunity is suppressed by the administration of colchicine.</p>

Keywords: Colchicine, Humoral immunity, Immune organs