IMPACT OF RHIZOSPHERE ANTAGONISTIC BACTERIA AND UREA FERTILIZER ON ROOT KNOT NEMATODE (MELOIDOGYNEINCOGNITA) UNDER GREEN HOUSE CONDITION

Authors: S. Ketabchi, H. Charehgani, S. Majzoob
Journal: Journal of Animal and Plant Sciences (JAPS)
ISSN: 1018-7081 (Print), 2309-8694 (Online)
Volume: 26  Issue: 6  Pages: 1780-1786
Year: 2016
DOI: NA
URL: https://doi.org/NA
Publisher: Pakistan Agricultural Scientists Forum

Abstract:
<p>The effect of rhizosphere bacteria on activity of root-knot nematode (<em>Meloidogyne incognita</em>) was investigated under laboratory and greenhouse conditions. Rhizosphere bacteria were isolated from roots of plants infected with root knot nematode. The effect of the rhizosphere bacteria on second stage juvenile mortality of&nbsp;<em>M. incognita&nbsp;</em>under laboratory conditions was studied after 24, 48 and 72 hs. Three bacterial isolates,&nbsp;<em>Serratia&nbsp;</em>sp.,<em>&nbsp;Pseudomonas fluorescens</em>CHA0, and&nbsp;<em>Pseudomonas putida</em>, that caused the higher mortality of second stage juvenile, were used in greenhouse experiment. They were evaluated with and without application of 50 mg urea fertilizer per kg of soil, on tomato (<em>Solanumly copersicum</em>) infected with&nbsp;<em>M. incognita</em>. All treatments had positive effect on plant growth parameters, and decreased the nematode-related parameters, such as number of gall, egg and egg mass, as well as the reproduction factor. Moreover, application of&nbsp;<em>Serratia</em> sp. in combination with urea fertilizer had the greatest effect, as compared to other treatments. The experiments were repeated and similar results were obtained.</p>

Keywords: Nitrogen, Pseudomonas fluorescens CHA0, Pseudomonas putida, Tomato, Serratia sp