EFFECT OF L-CYSTEINE ON POST THAW QUALITY OF NILI RAVI BUFFALO (BUBALUS BUBALIS) BULL SPERMATOZOA CRYOPRESERVED IN TRIS CITRIC ACID EXTENDER Authors: F. Wadood, M. Aleem, A. Ijaz, N. Ahmad, M. S. Yousaf, D. H. Mughal, A. Javid 8and S. A. Mahmood Journal: Journal of Animal and Plant Sciences (JAPS) ISSN: 1018-7081 (Print), 2309-8694 (Online) Volume: 25 Issue: 1 Pages: 301-303 Year: 2015 DOI: NA URL: https://doi.org/NA Publisher: Pakistan Agricultural Scientists Forum Abstract:

This study was designed to determine effect of various concentrations of L- cysteine on frozen-thaw semen quality of Nili Ravi buffalo. Single ejaculate of four buffalo bulls (Nili-Ravi) was collected at weekly intervals for 5 weeks. Ejaculates of acceptable motility (≥70%) were pooled and semen was diluted by tris citric acid extender (TCAE; 300 mosm/kg osmotic pressure and ~7.0 pH) having 4 levels of L-cysteine (0.0, 2.0, 5.0 and 8.0 mM). Semen was equilibrated at 4˚C and packaged in 0.5 mL French straws with 20×106 spermatozoa/ straw and then frozen in liquid nitrogen. L-cysteine inclusion levels had non-significant (P> 0.05) effect on spermatozoa motility, viability, NAR, PMI and on lipid peroxidation. However, DNA damage in spermatozoa was significantly higher (P<0.05) in 8.0 mM L-cysteine. It was concluded that L-cysteine (2.0 and 5.0 mM) addition to cryopreserved semen had no significant effect on post thaw semen characteristics, except at 8 mM which adversely affected DNA integrity. This may be due to its toxicity at this concentration.

Keywords: cryopreservation, semen, spermatozoa, antioxidants, buffalo, L-cysteine