OPTIMIZATION OF ULTRASOUND-ASSISTED EXTRACTION CONDITIONS OF ANTIOXIDATIVE COMPONENTS FROM ARUNDINA GRAMINIFOLIA BY RESPONSE SURFACE METHODOLOGY BASED ON DPPH RADICAL SCAVENGING ASSAY Authors: X. F. Li, Y. T. Gao, X. X. Pang, X. F. Liu, W. Y. Li, Y. L. Zhang, B. Y. Fan Journal: Journal of Animal and Plant Sciences (JAPS) ISSN: 1018-7081 (Print), 2309-8694 (Online) Volume: 25 Issue: 3 Pages: 254-259 Year: 2015 DOI: NA URL: https://doi.org/NA Publisher: Pakistan Agricultural Scientists Forum Abstract:

Response surface methodology(RSM) was employed to optimize conditions for ultrasound-assisted extraction(UAE) of antioxidantive components from Arundinagraminifolia. 1,1-diphenyl-2-picryl-hydrazyl(DPPH) free radical scavenging assay was used to evaluate the activity of antioxidative components. Based on the single-factor test, we identified extraction time, ethanol volume fraction, extraction temperature and liquid-solid ratio as the main variables that influence DPPH free radical scavenging activity of A. graminifolia extract. The optimal conditions to achieve the maximum activity were determined as follows: extraction time 35 min, extraction temperature46ºC, ethanol volume fraction 74%and liquid-solidratio27 mL.g -1 . The scavenging rateof 78.71%was achieved under the optimal extraction conditions, which was well in agreement with the optimal predicted values (79.16%). This extraction method was simple and efficient and provided a method of sample preparation to determine DPPH radical scavenging activity of total antioxidative components from A. graminifolia. The research also provided a reference for full utilization of A. graminifolia and identified a technique to extract antioxidative components.

Keywords: Arundinagraminifolia, antioxidative components, ultrasound-assisted extraction, response surface methodology, DPPH radical scavenging gassay