EVALUATION OF DNA EXTRACTION METHOD FROM FORMALIN PRESERVED SKIN SAMPLES OF PANTHERA PARDUS FOR MOLECULAR GENETIC ASSESSMENT

Authors: S. S. Bibi, A. Rehman, R. A. Minhas, S. Janjua
Journal: Journal of Animal and Plant Sciences (JAPS)
ISSN: 1018-7081 (Print), 2309-8694 (Online)
Volume: 25  Issue: 4  Pages: 1196-1199
Year: 2015
DOI: NA
URL: https://doi.org/NA
Publisher: Pakistan Agricultural Scientists Forum

Abstract:
<p>Five different protocols,<em>&nbsp;viz.&nbsp;</em>Andleeb&nbsp;<em>et al.</em>&nbsp;(2012), Shrivastava&nbsp;<em>et al</em>. (2012), Phenol chloroform DNA extraction method, Roboscreen Kit method and Phenol chloroform triton method, were tested for DNA extraction from skin samples of Leopard (<em>Panthera pardus)&nbsp;</em>preserved in formalin over a period of two to five months. Among these procedures, the Phenol chloroform triton method was proved to be the most efficient protocol with 83% extraction rate and revealed 100% amplification of FCA026 and FCA043 loci. Keeping in views the extraction and amplification efficiency, Phenol chloroform triton method is recommended for molecular studies of formalin preserved skin samples of&nbsp;<em>Panthera pardus</em>.</p>

Keywords: Skin Tissue Samples, DNA Extraction, Polymerase Chain Reaction