BIOCHEMICAL CHANGES IN RESPONSE TO SALINITY IN CHICKPEA (CICER ARIETINUM L.) DURING EARLY STAGES OF SEEDLING GROWTH

Authors: M. Arefian, S. Vessal, A. Bagheri
Journal: Journal of Animal and Plant Sciences (JAPS)
ISSN: 1018-7081 (Print), 2309-8694 (Online)
Volume: 24  Issue: 6  Pages: 1849-1857
Year: 2014
DOI: NA
URL: https://doi.org/NA
Publisher: Pakistan Agricultural Scientists Forum

Abstract:
<p>Salinity is a serious abiotic stress, causing oxidative stress. Various biochemical parameters in chickpea genotypes were considered under varied NaCl concentrations (0, 8 and<a id="OLE_LINK15" name="OLE_LINK15"></a><a id="OLE_LINK16" name="OLE_LINK16"></a>&nbsp;12 dS.m-1<span dir="rtl">&nbsp;</span><span dir="rtl">(</span><span dir="ltr">&nbsp;</span>. This experiment was done as factorial arrangement (genotype &times; salt concentration &times; time) in a completely randomized design. Samples were&nbsp;<a id="OLE_LINK3" name="OLE_LINK3"></a><a id="OLE_LINK4" name="OLE_LINK4"></a>collected at 21 and 28-day old seedlings (28-DOS). The results revealed that increasing salt concentration resulted in higher levels for malondialdehyde content; among genotypes,&nbsp;<a id="OLE_LINK25" name="OLE_LINK25"></a>MCC806&nbsp;with 2.2 and MCC760 with 0.7 had the highest and lowest amount, respectively. Proline and protein&nbsp;<a id="OLE_LINK7" name="OLE_LINK7"></a><a id="OLE_LINK6" name="OLE_LINK6"></a>contents were significantly higher in MCC544 by 27-fold&nbsp;<a id="OLE_LINK10" name="OLE_LINK10"></a><a id="OLE_LINK9" name="OLE_LINK9"></a>increase (for proline) and 30% (for protein) relative to control in 28 DAS at 12&nbsp;<a id="OLE_LINK43" name="OLE_LINK43"></a><a id="OLE_LINK42" name="OLE_LINK42"></a>dS.m-1 of salt. The leaf soluble carbohydrates also increased significantly in MCC544 and MCC760 compared with the others. The minimum decline of electrolyte leakages (6%) was belonged to MCC760 while MCC806 genotypes showed the highest decrease rate (more than 20%). Total leaf chlorophyll content decreased in all genotypes during the stress. However, morphological damages in MCC544 and MCC760 genotypes were less in 28-DOS at 8 and 12 dS.m-1 NaCl, respectively. Overall, proline and leaf soluble carbohydrates were more consistent with salt tolerance responses of the genotypes, and 2 weeks after stress initiation (28-DOS) could be a critical stage for screening the genotypes.</p>

Keywords: Chickpea, Proline, Salinity, Total chlorophyll, Total soluble protein