A SIMPLE METHOD FOR PRODUCING AFLATOXIN B1 ON RICE MEDIUM FOR USE IN EXPERIMENTAL ANIMAL FEEDS Authors: A. W. Yunus, J. Böhm Journal: Journal of Animal and Plant Sciences (JAPS) ISSN: 1018-7081 (Print), 2309-8694 (Online) Volume: 21 Issue: 2 Pages: 303-304 Year: 2011 DOI: NA URL: https://doi.org/NA Publisher: Pakistan Agricultural Scientists Forum Abstract:

A simplification and standardisation of the existing methods to produce aflatoxin B1 (AFB1) on rice medium is described. As per conventional method, rice were first soaked in 1 ml water/2 g of rice for 2 hr, autoclaved, and then inoculated with Aspergillus flavus. During different stages of incubation, water was added according to different regimens. It was concluded that 1 ml water/100 g of rice at 24 to 36 hr of incubation is the maximum amount of water that could be added for optimal AFB1 production.

Keywords: Aflatoxin, Rice, Aspergillus flavus