PRODUCTION OF GOAT POX VIRUS VACCINE FROM A LIVE ATTENUATED GOAT POX VIRUS STRAIN Authors: F. Abbas, F. A. Khan, A. Hussain, F. Ahmed, M. Ahmed, S. Ahmed, M. Z. Ahmed, M. A. Attique, A. Wadood, M. K. Taj. Journal: Journal of Animal and Plant Sciences (JAPS) ISSN: 1018-7081 (Print), 2309-8694 (Online) Volume: 20 Issue: 4 Pages: 315-317 Year: 2010 DOI: NA URL: https://doi.org/NA Publisher: Pakistan Agricultural Scientists Forum Abstract:

Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days.   Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers.   Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation.  TCID50 calculated for the pool virus was 10 5.5/ ml.  Vaccine was found free from any bacterial and mycoplasma contamination. All mice and guinea pigs were found healthy after 21days post vaccinal administration.  Vaccinated goats challenged after 14 days with 1 ml of virulent goat pox virus (TCID50 104.5/ml) did not show any unwanted reaction while controls challenged with 1 ml of virulent goat pox virus (TCID50 10 3.5/ml)  showed pox lesions and rise in body temperature. The main objective of this study was to produce and study the efficacy of this vaccine.

Keywords: attenuated, goat pox virus, confluent, monolayer, virulent, challenge