DETERMINATION OF LETHAL AND FEEDING DETERRENT ACTIVITIES OF SAPONIN FROM PHALERIA MACROCARPA AGAINST POMACEA MACULATA - A. R. Roonjho; R. Muhamad; D. Omar (Journal of Animal and Plant Sciences, 2020)
Apple snail is one of the major pest of rice crop and saponin proved to be the most promising bioactive compound to control it. This study was carried out to quantify saponin from God’s Crown, Phaleria macrocarpa and to evaluate its efficacy against the biological activities of black apple snail, Pomacea maculata. Fruits, leaves and stem-barks of P. macrocarpa were quantified for saponin using HPLC. The toxicity of leaf and fruit crude extracts was evaluated through mortality and feeding deterrent bioassays using complete randomized design and data were analyzed by ANOVA for LSD test. The highest saponin contents 24.67 ppm was detected in fruits followed by 22.67 ppm in leaves and 5.94 ppm in stem-bark. Bioassays showed the highest mortality percentage (44%) after 24 hours exposure at the concentration of 1000 ppm of a leaf extract followed by 36% and 28% @ 750 and 1000 ppm of leaf and fruit extracts, respectively. After the exposure of 48 hours, mortality percentage increased to 100% @ 1000 and 750 ppm of both crude extracts while the mortality percentage recorded at the concentration of 500 ppm of leaves and fruits were 56% and 52% respectively. Mortality percentage at the concentration of 500 ppm was increased to 80% and 68% in leaf and fruit extracts after exposure of 72 hours, respectively. In terms of feeding deterrent, 1000, 750 and 500 ppm concentration of both crude extracts were not significantly different from the positive control niclosamide (p>0.05). The results obtained from the study revealed that saponin extracted from fruits and leaves of P. macrocarpa has a potential to control black apple snails.
Keywords: journal article; Phaleria macrocarpa; Pomacea maculata; HPLC; botanical molluscicides; saponins