EFFECTS OF SYNBIOTIC FROM NONI LEAF EXTRACT SUPPLEMENTATION ON SURVIVAL, GROWTH, AND RESISTANCE TO Vibrio harveyi IN THE WHITELEG SHRIMP, Litopenaeus vannamei - Thi Cam Tu Phan; Truong Giang Huynh; Thi Kim Lien Nguyen; Thi Thanh Hien Tran (Journal of Animal and Plant Sciences, 2026)
This study assessed the effects of noni leaf and Lactobacillus plantarum CMT1 on survival, growth, and resistance to Vibrio harveyi of whiteleg shrimp, Litopenaeus vannamei, by improving digestive function and immunity. The experiment was conducted in a recirculating aquaculture system using a completely randomized design with five treatments and three replicates per treatment. One thousand five hundred healthy shrimp were assigned to 5 diet groups for a 60-day feeding period, including a control (commercial feed), diet 1 (commercial feed + 0.5% noni leaf), diet 2 (commercial feed + 1% noni leaf), diet 3 (commercial feed + 0.5% noni leaf + 108 CFU kg-1 of L. plantarum CMT1), and diet 4 (commercial feed + 1% noni leaf + 108 CFU kg-1 of L. plantarum CMT1). One-way ANOVA analysis showed that, compared with the control diet, other diets greatly enhanced growth indices of shrimp, with the highest growth observed in diet 3 (p ≤ 0.05). Supplementation with diets 1, 2, 3, and 4 improved the abundance of Lactobacillus spp. and decreased Vibrio spp. in the shrimp intestine. Shrimp fed diets 1 and 3 showed significantly higher amylase activity than those in the control and in diets 2 and 4. In addition, shrimp fed diet 3 group showed greatly enhanced survival, increasing from 78.7% to 88.3%, and improved immune parameters after Vibrio harveyi challenge compared to other experimental diets and the control. In summary, the administration of 0.5% noni leaf and 108 CFU kg-1 of L. plantarum CMT1 increased growth performance, improved digestive function, and immunity system as well as promoted the survival rate against V. harveyi in shrimp.
Keywords: journal article; Morinda citrifolia; leaf extract; Litopenaeus vannamei; synbiotic; digestive enzyme