MORPHOLOGICAL AND MORPHOMETRICAL STUDIES OF TRYPANOSOMA DANILEWSKYI STRAIN FCC-1. - M. S. Ahmed; K. Shafiq H. Ali; F. Ollevier (Journal of Animal and Plant Sciences, 2013)
Morphological and morphometrical study was conducted on blood stream forms of Trypanosoma danilewskyi strain F Cc-1, isolated from laboratory-infected single breed juvenile common carp. A group of ten fish was intraperitoneally inoculated with 1000 live trypanosomes per fish. At the peak of parasitemia thin blood smears were prepared on precleaned glass slides on days 20, 30, 40 and 50 post infections (p.i). Blood smear was air dried, fixed with methanol and stained with May-Grünwald Giemsa stain. Stained trypanosomes were examined under light microscope at X 1000 magnification in the immersion oil. Morphology of trypanosome was recorded with a camera mounted on the microscope. From well stained slides 200 good specimens of T. danilewskyi FCc-1 were selected. For morphometric parameters, linear measurements in microns and nuclear area in square microns were taken by using Videoplan ( Image Analysis System Kontron Bildanalyse, Germany) . Morphometric measurements of T. danilewskyi strain FCc-1 were compared with reference strain of T. danilewskyi (Caa-1) already described earlier. The data revealed that T. danilewskyi strains FCc-1 and Caa-1 are the same. passages), isolated from experimentally infected juvenile
Keywords: journal article; morphometry; T. danilewskyi strain FCc-1; common carp; species identification; reference strain Caa-1