EFFECTS OF DIFFERENT HORMONE COMBINATIONS ON SUPEROVULATION IN RIVER BUFFALOES - Guang-Sheng Qin; Ming-Tang Chen; Xian-Wei Liang; Xiu-fang Zhang; Chun-Ying Pang; Sheng-Ju Wei; Fen-xiang Huang; He-sheng Jiang (Journal of Animal and Plant Sciences, 2012)
This study was conducted to evaluate the effects of different hormone combinations with FSH, PGC, LHRH-A3 and LH on superovulation in river buffaloes. Thirty-five heads of river buffaloes were divided into six groups as follows: Group I, FSH (Japan, total doses 26 AU) + PGc (Shanghai, 0.6 mg); Group II, FSH (Japan, total doses 26 AU) + PGc (Shanghai, 0.6 mg) + LHRH-A3 (Made in Ningbo, 50 μg) ; Group III, FSH (Beijing, total doses 20 mg ) + PGc (Shanghai, 0.6 mg) + LHRH-A3 (Ningbo, 50 μg) ; Group IV, FSH (Beijing, 20 mg) + PGc (Shanghai, 0.6 mg) + LH (Ningbo, 50 μg); Group V, FSH (Canada, 800 mg) + PGc (Shanghai, 0.6 mg); Group VI, FSH (Canada, 800 mg) + PGc (Shanghai, 0.6 mg) + LHRH-A3 (Ningbo, 50 μg). The results showed that superovulation rate was 97.14% (34/35). There were 8.71 mature follicles per head in superovulation (296/34). The average number of CL was 5.0 (170/34). The average ovulation rate was 57.3% (170/296). The average number of embryo collection was 2.72. Average transferable embryos were 1.33 (24/18). Recovery rate and transferable rate were 39.84% and 48.98 %, respectively. The mean number of CL in Group II (6.86±5.96) and the ovulation rate in Group VI (76.92 %) were the highest among the six groups. The results showed that the ovulation rate in Group VI with LHRH-A3 was higher than those in the other groups without LHRH-A3 (12.12%) and that with LH treatment (28.64%), respectively.
Keywords: journal article; River buffalo; Superovulation; FSH; LHRH-A3