17 M. H. Lashari M. Bibi U. Farooq F. Afzal A. Ali M. Safdar 2 M. S. Akhtar A. A. Farooq4 S. Masood5 M. Ayaz M. I. Khan Journal of Animal and Plant Sciences JAPS 20202020/10/03 31 2 625-629 1018-7081 https://doi.org/10.36899/JAPS.2021.2.0251 <p>The purpose of this study was to identify the seroprevalence of&nbsp;<em>Toxoplasma gondii</em>&nbsp;(<em>T. gondii</em>) and hematological alterations in captive pheasant species of Bahawalpur Zoo, Bahawalpur, Pakistan. The blood samples of 100 birds belonging to three different species&nbsp;<em>viz</em>. ring-necked pheasant (<em>Phasianus colchicus</em>) (n=46), green pheasant (<em>Phasianus versicolor</em>) (n=40), and silver pheasant (<em>Lophura nycthemera</em>) (n=14) were analyzed through Latex Agglutination Test (LAT) and Enzyme Linked Immunosorbent Assay (ELISA). Seroprevalance through LAT in ring-necked, silver and green pheasants was 10.86%, 7.14% and 7.5%, and through ELISA was 32.60%, 22.5% and 14.28%, respectively. Adults and males had higher prevalence as compared to their counterparts, though, statistically nn-significant (P&ge;0.05). In all the studied species, hematological results revealed that mean values of Hb and TEC were higher (P&le;0.05) in non-infected birds as compared to infected ones. This is a preliminary study of a kind and needs further research with large number of sample and population across the country.</p> Toxoplasma gondii, ELISA, LAT, Seroprevalance, Oocytes, Hematology parameters Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=VS-19-0091