17 A. Murtaza 1# S. Ullah1# I. Tauseef K. S. Haleem M. Jamal J. Gu Asadullah S. Ayaz M. Shafiq N. A. Khan S. U. Rahman4 Journal of Animal and Plant Sciences JAPS 20202020/11/09 31 3 698-707 1018-7081 https://doi.org/10.36899/JAPS.2021.3.0260 <p>Antimicrobial resistance is increasingly reported worldwide, however, there is scarcity of data from Pakistan. Particularly, data from food-producing animals and their products is totally lacking. Here, we report on the ESBL-genotypes, integron types, insertion sequence, plasmid replicon typing and susceptibility to commonly used-antimicrobials of&nbsp;<em>E. coli</em>&nbsp;recovered from poultry-meat in live bird market of District Mardan, Khyber Pakhtunkhwa, Pakistan. Random&nbsp;<em>E. coli</em>&nbsp;isolates were picked up for screening of ESBL-production and -genotype, antimicrobial resistance profile through disc diffusion method and integron and plasmid replicon typing. Of the 33&nbsp;<em>E. coli&nbsp;</em>multidrug resistant isolates (resistant to tetracycline, ampicillin, cefotaxime and novobiocine), 28 (phylogroup B2=11, A=9 and D=8) were ESBL producers harboring&nbsp;<em>bla_CTX-M&nbsp;</em>(<em>bla_CTXM-9</em>₌25 and<em>&nbsp;bla_CTXM-1</em>=3,&nbsp;<em>bla_CTXM-15</em>=3), while, none of them were carrying&nbsp;<em>bla_SHV</em>&nbsp;or&nbsp;<em>bla_TEM</em>. Majority of these ESBL producers were carrying class-1 integron (n=21), although 11 isolates were carrying IS<em>CR1</em>, which found to be linked with&nbsp;<em>bla_CTX-M</em>&nbsp;among 5 isolates (45.5%). PCR based plasmid replicon typing revealed that FrepB replicon was found most dominantly and carried by 27 isolates followed by B/O replicon. This suggests that poultry raw meat may contribute to spread of ESBL-producing&nbsp;<em>E. coli</em> or ESBL- genes.</p> ESBL; food-producing animals; integron; replicon typing; ISCR; multidrug resistant; E Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=VS-19-0075