17 A. Ahmed M. Ayaz D. H. Mughal A. Rehman M. H. Mushtaq M. S. Yousaf B. U. Din A. Riaz Journal of Animal and Plant Sciences JAPS 20202020/03/25 30 3 612-618 1018-7081 https://doi.org/10.36899/JAPS.2020.3.0072 <p>This study was designed to investigate the effect of L-lysine on post-thaw quality of Nili-Ravi buffalo bull semen. Twenty eight ejaculates were collected from four Nili-Ravi buffalo bulls. Semen samples were pooled and split into five equal aliquots and diluted in Tris-citric acid extender containing different concentrations of L-lysine (0.25, 0.5, 1 and 2mM). As a control, semen was extended without addition of L-lysine. The semen samples were cryo-preserved in liquid nitrogen. Post-thaw semen assessment revealed that sperm motility, viability, plasma membrane integrity, acrosomal integrity, mitochondrial integrity and DNA integrity were significantly improved (P &le; 0.05) in 2mM L-lysine supplemented group as compared to control. For other groups supplemented with L-lysine; post-thaw motility, acrosomal integrity and mitochondrial integrity were also improved (P &le; 0.05) for 1mM treated group as compared to control. DNA integrity was improved significantly by all L-lysine treated groups as compared to control. Malondialdehyde concentration was higher (P &le; 0.05) in control group as compared to L-lysine treated groups. Significantly higher rate of catalase was recorded for 1mM and 2mM L-lysine treated groups as compared to control. In conclusion, the supplementation of 2mM L-lysine in semen extender can be used for cryopreservation of Nili-Ravi buffalo bull semen.</p> Nili-Ravi, Semen, Cryopreservation, L-lysine, Antioxidant Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=IBC-19-044