17 N. George G. Siddiqui F. Muhammad Z. George Journal of Animal and Plant Sciences JAPS 20212021/08/07 31 5 1530-1536 1018-7081 https://doi.org/10.36899/JAPS.2021.5.0355 <p>DNA barcoding is considered an effective tool for the identification of organisms on the molecular level. The present study aimed to focus on DNA barcoding of<em>&nbsp;Telescopium telescopium</em>&nbsp;(Gastropod) using mitochondrial cytochrome C oxidase subunit I (COI) gene and to correlate it with morphologically identified species found along the coast of Karachi, Pakistan. Our results of BLAST analyses show 95% similarity with the partial sequence of&nbsp;<em>T. telescopium</em>&nbsp;reported from Goa and Kerala, India whereas, the genomic distance of mt-COI gene among the&nbsp;<em>T. telescopium&nbsp;</em>population is 0% to 45%.&nbsp;<em>Telescopium telescopium</em>&nbsp;of Karachi, Pakistan showed 0% evolutionary divergence with the&nbsp;<em>T. telescopium</em>&nbsp;of Goa and Kerala, India and maximum distance 45% with&nbsp;<em>T. telescopium</em>&nbsp;of Bangladesh. This investigation confirmed the morphological and molecular identification of&nbsp;<em>T. telescopium</em> for the first time from Karachi, Pakistan.</p> DNA barcoding, mt-COI gene sequence, Telescopium telescopium, Molecular phylogeny Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=Fish-20-0012