17 H. Hong S. Cho K. Niu J. Kim Y. J. Ham W.D. Lee S. K. Kim1 Journal of Animal and Plant Sciences JAPS 20202020/12/07 31 3 782-790 1018-7081 https://doi.org/10.36899/JAPS.2021.3.0290 <p>The present study aimed to optimize medium component which maximize antibacterial spectrum and cell yield of&nbsp;<em>Lactobacillus plantarum</em>&nbsp;SK1305 at the same time.&nbsp;<em>L. plantarum</em>&nbsp;isolated from Korean traditional pickled pepper was used and its antibacterial activity against&nbsp;<em>Salmonella typhimurium</em>,&nbsp;<em>Listeria monocytogenes</em>,&nbsp;<em>Burkholderia</em>&nbsp;sp.,&nbsp;<em>Enterococcus faecalis</em>,&nbsp;<em>Salmonella gallinarum</em>, and&nbsp;<em>Staphylococcus aureus</em>&nbsp;were investigated. Alteration of medium components was performed using a fractional factorial design and weighted standardization method was applied in optimization of medium components. Inversed probability of medium component effect was employed in weighting. Total of 15 medium ingredients: sucrose, maltose, molasses, yeast extract, corn steep liquor, whey, K₂HPO₄, MnSO₄, MgSO₄, tween 80, NaCl, CH₃COONa, C₆H₁₁NO₇, Na₂SO₄, and FeSO4, were used as variables. The significant positive effects on cell yield were found for maltose and FeSO₄. The effects of ingredients on the antibacterial spectrum were summarized using weighted standardization, and sucrose and CH₃COONa were determined as the most important ingredients. These results provide useful information about medium ingredients for the improvement of the antibacterial spectrum of&nbsp;<em>L. plantarum</em> SK1305.</p> Lactobacillus plantarum; medium ingredients; antibacterial activity; cell yield; fractional factorial design Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=Biot-19-0051