17 N. Akhtar1 A. Kiran Sajid-ur-Rehman M. Kausar M. Khan U. Saleem T. Mahmood Journal of Animal and Plant Sciences JAPS 20202020/11/11 31 3 877-887 1018-7081 https://doi.org/10.36899/JAPS.2021.3.0285 <p>Development^&nbsp;of^&nbsp;rust^&nbsp;resistant^&nbsp;wheat^&nbsp;cultivars^&nbsp;to^&nbsp;meet^&nbsp;ever-increasing^&nbsp;wheat^&nbsp;production^&nbsp;targets^&nbsp;for^&nbsp;national^&nbsp;food^&nbsp;security^&nbsp;in^&nbsp;climate^&nbsp;change^&nbsp;scenario^&nbsp;is^&nbsp;currently^&nbsp;at^&nbsp;high^&nbsp;priority^&nbsp;in^&nbsp;Pakistan.^&nbsp;Focusing^&nbsp;on^&nbsp;this,^&nbsp;here^&nbsp;we^&nbsp;report^&nbsp;the^&nbsp;screening^&nbsp;of^&nbsp;40^&nbsp;wheat^&nbsp;genotypes^&nbsp;including^&nbsp;advanced^&nbsp;breeding&nbsp;lines^&nbsp;and^&nbsp;commercial^&nbsp;varieties^&nbsp;through^&nbsp;PCR^&nbsp;based^&nbsp;DNA^&nbsp;markers^&nbsp;to^&nbsp;detect^&nbsp;genes^&nbsp;conferring^&nbsp;resistance&nbsp;against^&nbsp;different^&nbsp;rust^&nbsp;races.^&nbsp;In^&nbsp;this^&nbsp;study^&nbsp;22^&nbsp;SSR/STS^&nbsp;markers^&nbsp;were^&nbsp;used^&nbsp;which^&nbsp;included^&nbsp;nine^&nbsp;leaf^&nbsp;rust^&nbsp;(Lr19,^&nbsp;Lr22a,^&nbsp;Lr27,^&nbsp;Lr28,^&nbsp;Lr34,^&nbsp;Lr35,^&nbsp;Lr37,^&nbsp;Lr46^&nbsp;and^&nbsp;Lr67),^&nbsp;eight^&nbsp;stripe^&nbsp;rust^&nbsp;(Yr5,^&nbsp;Yr15,^&nbsp;Yr17,^&nbsp;Yr18,^&nbsp;Yr26,^&nbsp;Yr29,^&nbsp;Yr30^&nbsp;and^&nbsp;Yr46)^&nbsp;and^&nbsp;seven^&nbsp;stem^&nbsp;rust^&nbsp;resistance^&nbsp;genes^&nbsp;(Sr2,^&nbsp;Sr22,^&nbsp;Sr24,^&nbsp;Sr25,^&nbsp;Sr26,^&nbsp;Sr38^&nbsp;and&nbsp;Sr39).^&nbsp;The^&nbsp;results^&nbsp;revealed^&nbsp;that^&nbsp;allelic^&nbsp;frequency^&nbsp;for^&nbsp;rust^&nbsp;resistance^&nbsp;genes^&nbsp;(Lr,^&nbsp;Yr^&nbsp;and^&nbsp;Sr)^&nbsp;was^&nbsp;maximum^&nbsp;(70.4%)^&nbsp;in^&nbsp;three^&nbsp;advanced^&nbsp;wheat^&nbsp;lines^&nbsp;namely^&nbsp;V-9452,^&nbsp;V-11153^&nbsp;and&nbsp;NNG-3.^&nbsp;However,^&nbsp;minimum^&nbsp;frequency^&nbsp;(51.9%)^&nbsp;was^&nbsp;found^&nbsp;in^&nbsp;25^&nbsp;genotypes.^&nbsp;Three&nbsp;advanced^&nbsp;lines^&nbsp;NNG-3,^&nbsp;V-9432^&nbsp;and^&nbsp;V-11153^&nbsp;had^&nbsp;maximum^&nbsp;number^&nbsp;of^&nbsp;rust^&nbsp;resistance^&nbsp;genes^&nbsp;among^&nbsp;all^&nbsp;the^&nbsp;reported^&nbsp;wheat^&nbsp;genotypes&nbsp;which^&nbsp;indicated^&nbsp;narrow^&nbsp;genetic^&nbsp;base^&nbsp;of^&nbsp;Pakistani^&nbsp;wheat^&nbsp;cultivars^&nbsp;for^&nbsp;rust^&nbsp;resistance.^&nbsp;Therefore,^&nbsp;it^&nbsp;is&nbsp;suggested^&nbsp;that^&nbsp;newly^&nbsp;discovered^&nbsp;rust^&nbsp;resistance^&nbsp;genes^&nbsp;especially^&nbsp;Lr^&nbsp;and^&nbsp;Yr^&nbsp;should^&nbsp;be^&nbsp;used^&nbsp;along^&nbsp;with^&nbsp;existing^&nbsp;genes^&nbsp;for^&nbsp;effective^&nbsp;and^&nbsp;durable^&nbsp;rust^&nbsp;resistance^&nbsp;in^&nbsp;new^&nbsp;wheat varieties.</p> Gene, heat map, DNA markers, PCR, SSR Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=Biot-19-0041