17 I. Gull A. Noreen M. S. Aslam Z. Abbas1 M. A. Athar Journal of Animal and Plant Sciences JAPS 20192019/02/01 29 1 109-116 1018-7081 N/A <p>Storage of germplasm using synthetic seed technology is useful to avoid somaclonal variations as well as to cut down the cost and labor of germplasm maintenance under&nbsp;<em>in vitro</em>&nbsp;culturing conditions. In the present study, different parameters that affect the viability and regrowth ability of&nbsp;<em>Rosa indica&nbsp;</em>germplasm stored as synthetic seeds were optimized. The compact, firm, transparent synthetic seeds showing maximum regeneration rate (100 %) were prepared and stored in three different types of air tight storage vessels (glass jars, polypropylene tubes and plastic bags) at 4 &deg;C and 25 &deg;C for various time durations (0-8 weeks). No significant difference in regeneration frequency of&nbsp;<em>Rosa indica&nbsp;</em>synthetic seeds was recorded at different storage conditions. However, the storage temperature significantly influenced the viability and regrowth ability of synthetic seeds. The survival rate (80%) of encapsulated explants was observed up to 8 weeks at 4 &deg;C and declined with elapse of storage duration. Facilitated extrusion of shoot from gel seed and pre-treatment of synthetic seeds with 100 mM KNO3for 5 minutes improved the regeneration frequency while mechanical removal of gel bead resulted in complete loss of explant viability.</p> synthetic seed, viability, Rosa indica, nodal segments Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=Biot-16-0049