17 S. Ambreen Z. Mehmood K. Mehmood A. Sajjad A. Mushtaq S. Rizwan U. Jabeen F. Bashir F. Behlil Journal of Animal and Plant Sciences JAPS 20222022/04/01 32 2 526-531 1018-7081 http://doi.org/10.36899/JAPS.2022.2.0450 <p>20 honey samples from district, Balochistan (Pakistan) were studied for their physiochemical, biochemical and antioxidant potential. During the study, pH, ash content, moisture, total fat, sugar content, reducing and non-reducing sugars, vitamin C, Vitamin A, flavonoids, polyphenols and % DPPH scavenging activity were monitored. The pH of the samples was between4.0 - 5.0, having mean&plusmn;SD values of 4.5 &plusmn; 0.1. The moisture and ash content had an average value of 17.4 &plusmn; 0.7 g/100g and 0.142 &plusmn; 0.0 g/100g respectively. The fat content had range of values from minimum to maximum 0.12 - 0.49 g/100g with mean&plusmn;SD of 0.297 &plusmn; 0.11. Total sugar content varied between 66.2 - 71.8 g/100g with mean and S.D 68.88 &plusmn; 1.7. The sucrose content ranged between 0.60 - 4.28 g/100g with mean&plusmn;SD 1.93 &plusmn; 0.8 while average value of fructose and glucose values were 36.84g/100g &plusmn; 0.9 and 30.41g/100g &plusmn; 3.00 respectively. All the tested samples showed very low content of Vitamin A while Vitamin C and flavonoids showed the average values of 112.45 &plusmn; 9.8 mg/kg and 43.0 &plusmn; 2.0 mg CE/kg respectively and polyphenols showed the average value of 748.76 &plusmn; 31.6 mg GAE/kg with 6057.5 - 887.2 mg GAE/kg min to max values. The % DPPH scavenging at 40mg/ml showed the values 61.8 - 75.7 min to max with the average of 70.3 &plusmn; 0.9. All the tested samples lie within the limits of international standard but Sample P4 showed the best results among all the 20 honey samples.</p> Honey, composition, biochemical, physiochemical, Antioxidant, flavonoids, total Sugars Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=Bioch-20-0031