17 S. Yasmin M. S. Yousaf K. A. Majeed M. A. Rashid M. Numan R. Mustafa S. K. Tahir H. Zaneb H. Rehman Journal of Animal and Plant Sciences JAPS 20222022/03/15 32 2 336-344 1018-7081 https://doi.org/10.36899/JAPS.2022.2.0430 <p>The objective of the present study was to examine the effects of&nbsp;<em>Catharanthus roseus</em>&nbsp;(<em>C. roseus</em>) extract (leaves and roots) on growth performance and microarchitecture of the small intestine in Cobb 500 broilers. One hundred and seventy-five, 1-day-old chicks were randomly divided into seven treatment groups (n = 25/group) with five replicates (n = 5/replicate). Birds in group 1 served as a control and were offered a corn soybean-based basal diet only. The birds in groups 2, 3, and 4 were fed the same diet supplemented with 0.05%, 0.1%, and 0.2% aqueous leaf extracts of&nbsp;<em>C. roseus</em>. Whereas, groups 5, 6, and 7 were provided the diet supplemented with 0.05%, 0.1%, and 0.2% aqueous root extracts of&nbsp;<em>C. roseus</em>, respectively, for 35 days. Supplementation of&nbsp;<em>C. roseus</em>&nbsp;extracts did not improve body weights, weight gain, feed intake, and feed conversion ratio. Relative weights of viscera remained unchanged except that of gizzard and pancreas which were lower (P &lt; 0.05) in 0.1% ALE supplemented birds. The microarchitectural attributes including villus height, width, surface area, crypt depth, and the ratio of villus height to crypt depth were higher (P &lt; 0.05) in the 0.05% ALE supplemented group. In conclusion supplementation of&nbsp;<em>C. roseus</em> extracts did not improve growth performance but improved gut histomorphometric parameters of the small intestine in broilers.</p> Phytobiotics, Poultry, Feed additive, Growth Performance, Intestinal Morphometry Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=AS-20-0093