ASSOCIATIONS BETWEEN PRL/RsaI POLYMORPHISM AND SOME PERFORMANCE TRAITS IN HOLSTEIN CATTLE REARED UNDER ORGANIC CONDITION

M. Ozdemir Z. Sonmez V. Aksakal ASSOCIATIONS BETWEEN PRL/RsaI POLYMORPHISM AND SOME PERFORMANCE TRAITS IN HOLSTEIN CATTLE REARED UNDER ORGANIC CONDITION

Journal of Animal and Plant Sciences

JAPS

2020

2020/11/09

31 3 900-905 1018-7081

https://doi.org/10.36899/JAPS.2021.3.0279

The aim of this study was to ascertain the RsaI polymorphism of the Prolactin (PRL) gene of 186 organically reared Holstein cattle by PCR-RFLP method, in order to reveal the genetic variation of the population, and to reveal the relationship between PRL/RsaI genotypes and some milk yield traits. In the study, the RsaI-/- genotype frequency, RsaI+/+ genotype frequency and RsaI-/+ genotype frequency of Holstein cattle were determined as 0.26, 0.22 and 0.52, respectively. The RsaI- gene frequency was 0.52, and the RsaI+ gene frequency was 0.48. Hardy-Weinberg genetic equilibrium test showed that the distribution of genotype frequencies was determined to be in genetic equilibrium. The relationships between PRL/RsaI polymorphism and the examined milk yield traits were not significant. As a result, an economic and safe method PCR-RFLP for the detection of genetic markers, was successfully used in the determination of the 3 genotypes (RsaI-/-, RsaI-/+, and RsaI+/+) of Prolactin gene in Holstein cattle reared under organic condition, and the results was similar with previously reported literature. Prolactin, PCR-RFLP, polymorphism, marker, cattle Pakistan Agricultural Scientists Forum

https://thejaps.org.pk/AbstractView.aspx?mid=AS-18-0138