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      <ref-type name="Journal Article">17</ref-type>
      <contributors>
        <authors>
          <author>Rana Yaser Arafat</author>
          <author>Saima</author>
          <author>Maqsood Akhtar</author>
          <author>Muhammad Naveed-ul-Haque</author>
          <author>Hifzulrahman</author>
        </authors>
      </contributors>
      <titles>
        <title>EFFECTS OF FEEDING CALCIUM SALTS OF FATTY ACIDS AND TWO LEVELS OF RUMEN UNDEGRADABLE PROTEIN ON PRODUCTION PERFORMANCE OF LACTATING NILI-RAVI BUFFALOES</title>
        <secondary-title>Journal of Animal and Plant Sciences</secondary-title>
        <alt-title>JAPS</alt-title>
      </titles>
      <dates><year>2026</year><pub-dates><date>2026/01/20</date></pub-dates></dates>
      <volume>36</volume>
      <number>1</number>
      <pages>298-306</pages>
      <isbn>1018-7081</isbn>
      <electronic-resource-num>https://doi.org/10.36899/JAPS.2026.1.0025</electronic-resource-num>
      <abstract>&lt;p&gt;Production performance of lactating&amp;nbsp;&lt;em&gt;Nili Ravi&lt;/em&gt;&amp;nbsp;buffalo can be improved with feeding of high rumen undegradable protein (RUP) and calcium salts of fatty acids (Ca-FA). Sixteen multiparous lactating&amp;nbsp;&lt;em&gt;Nili Ravi&lt;/em&gt;&amp;nbsp;buffaloes were arranged in a 4&amp;times;4 Latin square arrangement with (mean &amp;plusmn; SD) 11.2&amp;plusmn;0.76 kg/d of milk yield, 6.41&amp;plusmn;0.23% milk fat, 583&amp;plusmn;26 kg of body weight (BW) and 161&amp;plusmn;24 days in milk (DIM). The dietary treatments were: (1) LPLF-low RUP low fat (2) LPHF-low RUP high fat (3) HPLF-high RUP low fat and (4) HPHF-high RUP high fat. The designed diets provided low and high levels of RUP (27.4% and 38.6%) with low (3.5%) and high fat (4.9%). The duration of each period was 21 days and milk sampling was done twice weekly. Statistical significance among treatment means was evaluated using a p-value of ˂ 0.05. Increasing the RUP and fat supplies increased periodic BW and DMI (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;˂ 0.01) with no interaction effect of RUP &amp;times; fat (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;= 0.51) on BW. Milk yield was increased by 3.8% and 14.0% by increasing RUP and fat supplement respectively. The RUP &amp;times; fat interaction indicated that increase in milk yield was 51% higher when fat was supplemented with low RUP compared with the high RUP diet (RUP &amp;times; fat,&amp;nbsp;&lt;em&gt;P&lt;/em&gt;&amp;nbsp;&amp;lt; 0.01). Milk fat content was increased with the supplementation of fats by 22.0% and milk fat yield by 39.0% (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;&amp;lt; 0.01). Milk protein content and yield increased with the increasing RUP level by 10.8% and 15.7%, respectively. Milk lactose yield increased by 5.8% and 17.0% with increasing RUP and fat levels, respectively (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;&amp;lt; 0.01). Feed, nitrogen, and milk efficiencies including 4% fat corrected milk (FCM), energy corrected milk (ECM) and milk energy output were increased (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;˂ 0.01) with HF and HP treatments. Plasma urea nitrogen decreased with HP diet compared with LP diet (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;˂ 0.01), whereas the emission of enteric methane was decreased in HPHF group (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;˂ 0.01). In conclusion, the RUP fraction of 38.6% in concentrate and 300 gram of Ca-FA per animal per day increased the production performance of lactating&amp;nbsp;&lt;em&gt;Nili Ravi&lt;/em&gt; buffalo, whereas milk fat increased with fat supplementation and milk protein increased with high RUP supplies.&lt;/p&gt;</abstract>
      <keywords><keyword>Buffalo, DMI, rumen undegradable protein, rumen by pass fat, milk yield</keyword></keywords>
      <publisher>Pakistan Agricultural Scientists Forum</publisher>
      <urls><related-urls><url>https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-778</url></related-urls></urls>
    </record>
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