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      <ref-type name="Journal Article">17</ref-type>
      <contributors>
        <authors>
          <author>Murat Er</author>
          <author>Umair Ahsan</author>
          <author>Eren Kuter</author>
          <author>Hulisi Akçay</author>
          <author>Muhammad Shazaib Ramay</author>
          <author>Derya Merve Karagöz</author>
          <author>Mehmet Onur Takka</author>
        </authors>
      </contributors>
      <titles>
        <title>FERMENTATION QUALITY AND IN VITRO DIGESTIBILITY OF TOTAL MIXED RATION ENSILED WITH FRESH OLIVE CAKE</title>
        <secondary-title>Journal of Animal and Plant Sciences</secondary-title>
        <alt-title>JAPS</alt-title>
      </titles>
      <dates><year>2025</year><pub-dates><date>2025/09/30</date></pub-dates></dates>
      <volume>35</volume>
      <number>5</number>
      <pages>1335-1344</pages>
      <isbn>1018-7081</isbn>
      <electronic-resource-num>https://doi.org/10.36899/JAPS.2025.5.0114</electronic-resource-num>
      <abstract>&lt;p&gt;This study aimed to investigate the fermentation characteristics, aerobic stability, and&amp;nbsp;&lt;em&gt;in vitro&lt;/em&gt;&amp;nbsp;digestibility of the total mixed rations (TMR) ensiled with different amounts of fresh olive cake (OC). Three TMRs were ensiled with different OC percentages on DM basis; 0% OC (control); 10% OC (OC10), and 20% OC (OC20). For each group, 27 vacuum bags (silos; 81 bags in total) were prepared. Three bags from each group were opened on d 2, 4, 7, and 14 of ensiling to measure pH, and on d 21 and d 42 to evaluate the pH and aerobic stability. The remaining bags (9 from each group, 27 in total) were opened on d 60 to measure aerobic stability, fermentation quality (pH, organic acids, and NH&lt;sub&gt;3&lt;/sub&gt;-N), nutritional compositions, and&lt;em&gt;&amp;nbsp;vitro&lt;/em&gt;&amp;nbsp;digestibility. Increasing the OC inclusion in the TMRs increased the ether extract, neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) concentrations whereas, non-fibrous carbohydrates, water soluble carbohydrates, and total phenolic content decreased (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;&amp;le; 0.05). The control group had lower pH compared to other groups during the fermentation period (P&amp;le;0.05). No significant differences were seen among the treatments for lactic acid and acetic acid concentrations. However, propionic acid was greater in OC10 group compared to control (P&amp;le;0.05). Dry matter (DM) loss and aerobic stability were not different among the groups. Flieg score was greater in control group in comparison with that of OC20 group (P =0.016). Relative feed value (RFV) was greater in OC20 group than other groups (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;&amp;le; 0.001) whereas, control group had lower RFV than OC10 group (&lt;em&gt;P&lt;/em&gt;&amp;nbsp;&amp;le; 0.001).&amp;nbsp;&lt;em&gt;In vitro&lt;/em&gt;&amp;nbsp;digestibility parameters were lower in OC20 group in comparison with other groups (P&amp;le;0.001). Consequently, the results demonstrated that ensiling of TMR with OC had a good quality fermentation profile and feed value. However,&amp;nbsp;&lt;em&gt;in vitro&lt;/em&gt; nutrient digestibility was dramatically reduced when OC was used at 20% in TMR on a DM basis. Therefore, the inclusion of OC up to 10% in ruminant diets seems liable.&lt;/p&gt;</abstract>
      <keywords><keyword>Ensiled total mixed ration, olive cake, in vitro digestibility, fermentations characteristics</keyword></keywords>
      <publisher>Pakistan Agricultural Scientists Forum</publisher>
      <urls><related-urls><url>https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-31</url></related-urls></urls>
    </record>
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