17 Hina Tahir Saima Naveed Nisar Ahmad Muhammad Asim Tausif Burhan E Azam Sundas Qamar Saba Anwar Abdul Jabbar Muneer Ahmad Khan Muhammad Imran Muhammad Naveed-ul- Haque Journal of Animal and Plant Sciences JAPS 20252025/06/26 35 3 835-843 1018-7081 https://doi.org/10.36899/JAPS.2025.3.0072 <p>Feeding high starch diet is a common practice to increase milk production and similarly feeding sugars helps in improving milk fats. However, the extent to which trade-off between dietary starch and sugar content is possible for optimal milk production and fats is not fully explored in lactating Nili Ravi buffaloes. Twelve multiparous early-lactating Nili Ravi buffaloes (52 &plusmn; 37 d in milk, mean &plusmn;SD) received 3 treatments in a 3 &times; 3 Latin square design and each period consisted of 28 d. The dietary treatments contained starch and sugars in the following manner: 1) 28.7% starch, 2.61% sugar; 2) 25.9 % starch, 4.29% sugar; 3) 22.9% starch, 5.73% sugar on DM basis. All dietary treatments were iso-nitrogenous. Replacing dietary starch with sugar increased milk fat content, milk fat yield and 4% fat-corrected milk (FCM) linearly by 7.29, 13.8 and 11.9% respectively, however, milk yield was not affected by treatments. Similarly, energy-corrected milk (ECM) was also increased by 10.3% by the replacement of dietary starch with sugar. Body weight increased by 2% linearly, whereas, the body condition score remained unaffected as sugar replaced starch. There was a linear increase in plasma cholesterol concentrations, whereas plasma urea nitrogen, glucose, and triglyceride concentrations remained unaffected by increasing sugar. The marginal efficiency of NEL consumed for milk yield, 4% FCM and ECM increased linearly with increasing sugar. Feeding diets containing 22.9% starch and 5.37% sugar increased milk fat content, fat yield, and body weight of Nili Ravi buffalo without impacting on milk production and blood metabolites. Using sugar as a substitute for starch could be a practical option for producers without negatively impacting production.</p> Buffaloes, milk production, grain starch Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-15