17 M. A. Awad K. M. O. Ortashi A. Hagmusa B. Hagmusa E. M. Ibrahim G. Al-Sowygh H. Al-Shehri R. Ramadan Journal of Animal and Plant Sciences JAPS 20252025/02/18 35 1 49-57 1018-7081 https://doi.org/10.36899/JAPS.2025.1.0003 <p><span lang="EN-IN">This study aims to further explore the synthesis, characterization, encapsulation, and biomedical applications of&nbsp;<em>Adansonia digitata</em>&nbsp;Baobab nanoparticles.</span><span lang="EN-IN">Using a nano-precipitation technique, Gum Arabic and Polyvinyl alcohol were added to the nanoparticles that had been synthesized using the sonochemical process. Transmission electron microscopy was used to determine the physico-chemical properties of the synthesized and encapsulated nanoparticles, providing information about their morphology. Fourier Transform Infrared (FTIR) spectroscopy was employed to examine the chemical functional groups present in the samples.</span><span lang="EN-IN">The particle sizes of ADNPs and Cap-ADNPs were verified by dynamic light scattering (DLS) analysis. While encapsulated Cap-ADNPs had a greater average size of around 230 nm with a PDI of 0.311, the average particle size for ADNPs was approximately 94 nm with a PDI of 0.208. Tests were conducted on the antibacterial activity of ADNPs and Cap-ADNPs against a range of specific Gram-positive and Gram-negative bacteria as well as certain fungi. Additionally, the nanoparticles&#39; cytotoxicity toward human colon cancer cells (HCT-116) and human breast cancer cells (MCF-7) was assessed. With an IC50 of 73.6 mg/ml, ADNPs showed modest inhibitory action against HCT-116 cells; in contrast, Cap-ADNPs had a significantly greater impact, with an IC50 of 34.1 mg/ml. With an IC50 of 18.3 mg/ml, Cap-ADNPs have shown exceptional potency against MCF7 cells, whereas ADNPs had moderate inhibitory effects, with an IC50 of 64.7 mg/ml. According to preliminary findings, ADNPs and Cap-ADNPs have a great deal of promise to be effective therapeutic options in upgraded forms for use in bio-nanomedicine.</span></p> Adansonia digitata nanoparticles, nano-encapsulation, antimicrobial activity, cytotoxicity Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2024-JAPS-2215