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      <ref-type name="Journal Article">17</ref-type>
      <contributors>
        <authors>
          <author>Zahid Sharif Mirza</author>
          <author>Tariq Rashid</author>
          <author>Javairia Shafi</author>
          <author>Ahmed Shakeeb</author>
          <author>Abida Saeed</author>
        </authors>
      </contributors>
      <titles>
        <title>EFFECT OF TEMPERATURE ON PRODUCTION OF Moina brachiate AS LIVE FEED FOR Hypophthalmichthys molitrix FRY</title>
        <secondary-title>Journal of Animal and Plant Sciences</secondary-title>
        <alt-title>JAPS</alt-title>
      </titles>
      <dates><year>2025</year><pub-dates><date>2025/05/09</date></pub-dates></dates>
      <volume>35</volume>
      <number>3</number>
      <pages>681-688</pages>
      <isbn>1018-7081</isbn>
      <electronic-resource-num>https://doi.org/10.36899/JAPS.2025.3.0057</electronic-resource-num>
      <abstract>&lt;p&gt;The lack of mass culture techniques for&amp;nbsp;&lt;em&gt;Moina brachiate&lt;/em&gt;&amp;nbsp;limits its use as a sustainable live feed in aquaculture. Present study was conducted to culture&amp;nbsp;&lt;em&gt;M. brachiate&amp;nbsp;&lt;/em&gt;under indoor conditions at different temperatures and use it as live feed for&amp;nbsp;&lt;em&gt;H&lt;/em&gt;&lt;em&gt;ypophthalmichthys molitrix&lt;/em&gt;fry. The isolated&amp;nbsp;&lt;em&gt;M. brachiate&lt;/em&gt;&amp;nbsp;was cultured in the laboratory for 10 days in glass aquaria under two experimental treatments in which water temperature was maintained either at 28.5&amp;deg; &amp;plusmn; 1.5 &amp;deg;C (T1) or 23.5&amp;deg; &amp;plusmn; 1.5 &amp;deg;C (T2).&amp;nbsp;&lt;em&gt;Chlorella vulgaris&amp;nbsp;&lt;/em&gt;was used to feed&amp;nbsp;&lt;em&gt;M. brachiate&amp;nbsp;&lt;/em&gt;daily and the feed consumption was regularly monitored. Chemical quality parameters of the water were regularly monitored and kept within suitable range for propagationof&amp;nbsp;&lt;em&gt;Moina&lt;/em&gt;. At the end of the culture period, the maximum density of&amp;nbsp;&lt;em&gt;M. brachiate&lt;/em&gt;&amp;nbsp;was found in T2 (5.92 individuals/ ml). In T1 (83.05 &amp;plusmn; 1.39%) a decrease in density of&amp;nbsp;&lt;em&gt;M. brachiate&lt;/em&gt;&amp;nbsp;density was observed compared to its initial count. Consumption of&amp;nbsp;&lt;em&gt;C. vulgaris&lt;/em&gt;&amp;nbsp;by&amp;nbsp;&lt;em&gt;M. brachiate&amp;nbsp;&lt;/em&gt;was significantly high in T2 compared to T1 (90.54 &amp;plusmn; 3.69% and 69.32 &amp;plusmn; 11.81% respectively). Cysts of&amp;nbsp;&lt;em&gt;M. brachiate&lt;/em&gt;&amp;nbsp;were observed to be formed under drastic environmental conditions with hatching time of 7-8 days under optimum treatment. In the second phase of the experiment,&amp;nbsp;&lt;em&gt;M. brachiate&lt;/em&gt;&amp;nbsp;was used to feed&amp;nbsp;&lt;em&gt;H. molitrix&amp;nbsp;&lt;/em&gt;fry reared in glass aquaria and resulted in 86.0% survival of fry. The findings demonstrate the significant influence of temperature on&amp;nbsp;&lt;em&gt;M. brachiate&lt;/em&gt;&amp;nbsp;propagation and suggest that its culture at&amp;nbsp;&lt;strong&gt;23.5&amp;deg; &amp;plusmn; 1.5 &amp;deg;C water temperature with daily feeding of&amp;nbsp;&lt;em&gt;Chlorella vulgaris&lt;/em&gt;&lt;/strong&gt;&lt;strong&gt;,&amp;nbsp;&lt;/strong&gt;can be a viable live feed option for&amp;nbsp;&lt;em&gt;H. molitrix&amp;nbsp;&lt;/em&gt;fry in aquaculture.&lt;/p&gt;</abstract>
      <keywords><keyword>Live feed, Moina brachiate, Temperature, Survival rate, Feed Consumption</keyword></keywords>
      <publisher>Pakistan Agricultural Scientists Forum</publisher>
      <urls><related-urls><url>https://thejaps.org.pk/AbstractView.aspx?mid=2024-JAPS-1788</url></related-urls></urls>
    </record>
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