17 Muhammad Zubair-ul-Hassan Arsalan Syed Makhdoom Hussain Nisar Ahmad Bilal Ahmad Zeeshan Yousaf Muhammad Faisal Adan Naeem Journal of Animal and Plant Sciences JAPS 20242024/08/25 34 4 896-903 1018-7081 https://doi.org/10.36899/JAPS.2024.4.0774 <p>The purpose of this study was to analyze how the mineral digestibility of&nbsp;<em>Cirrhinus mrigala</em>&nbsp;was improved by the replacement of canola meal (CM) along with the supplementation of citric acid (CA) and phytase (PHY). A 90-day feeding trial was conducted in a completely randomized design. Sixteen test diets were formulated with different concentrations of CM (0%, 25%, 50%, and 75%), in the basal diet. Each concentration level was supplemented with four doses, one without supplementation, second with 2.5% CA, third with 750 FTUkg^-1&nbsp;PHY and fourth with combined supplementation (CA + PHY) to form sixteen test diets (T₁-T₁₆). Fifteen fingerlings (N = 720) were kept in each tank in triplicate. At the end of the trial, it was revealed that as the amount of CM in the diets increased, there was a significant (<em>P&le;</em>0.05) increase in mineral digestibility (Fe, Ca, Mg, K, Mn, Cu, P, Na, Cr, Zn and Al). The fish that consumed 50% CM-based diet supplemented with 2.5% CA and 750 FTUkg^-1&nbsp;PHY had the maximum apparent digestibility coefficient of minerals (K 73.76%, Ca 64.79%, Na 62.56%, P 73.11%, Cu 76.52%, Fe 77.14%, Mn 71.87%, Mg 71.42%). The results of this research suggest that supplementing a diet high in CM (50%) with 2.5 percent CA and 750 FTUkg^-1&nbsp;PHY is the most effective strategy to increase the digestibility of minerals in&nbsp;<em>C. mrigala</em> fingerlings.</p> Fish meal, substitution, aquaculture, plant meal, acidified phytase Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2023-JAPS-979