17 Syeda Maryam Fatima Wajiha Tahir Mahera Moin Humera Waheed Hanzala Khan Journal of Animal and Plant Sciences JAPS 20242024/10/22 34 5 1112-1118 1018-7081 https://doi.org/10.36899/JAPS.2024.5.0793 <p><em>Datura</em>&nbsp;is a flowering perennial herb, belonging to the family Solanaceae, is widely distributed in many countries including Pakistan, and other tropical and sub-tropical parts of the world. The present study explored the bioactivities of phytochemicals obtained from&nbsp;<em>Datura alba</em>&nbsp;n-hexane and n-butanol seed extracts. The seed extracts showed the presence of flavonoids, terpenoids and alkaloids while saponins were absent. The n-hexane extract showed 100% human salivary amylase and 19% snake venom PLA₂&nbsp;(svPLA₂) inhibition activity at examined lowest and highest concentrations respectively. It also exhibited mild (24% RSA) antioxidant activity at highest concentration (600 &micro;g/&micro;l) analyzed. On the other hand, salivary amylase inhibition and potent (80% RSA) antioxidant activity at 100 &micro;g/&micro;l concentration was observed in n-butanol extract. Interestingly, n-hexane extract showed snake venom protease while n-butanol extract showed svPLA₂&nbsp;enhancing activity and no venom anti-protease activity. The study demonstrated that both n-hexane and n-butanol extracts are natural sources of antioxidant and antidiabetic compounds. The n-hexane extract showed venom PLA₂ neutralization which is reported for the first time.</p> Traditional medicine;Datura alba; Anti-phospholipase A2; Anti-amylase; Antioxidant Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2023-JAPS-905