17 Mubashrah Munir Ishrat Fatima Beenish Aftab Ali Ahmed Sheikh Arshia Shabir Jamshaid Ahmad Muhammad Saeed Ahmed Journal of Animal and Plant Sciences JAPS 20232023/09/30 33 5 1126-1132 1018-7081 https://doi.org/10.36899/JAPS.2023.5.0706 <p><em>Stevia</em><em>rebaundiana</em>&nbsp;is containing sweet phytochemicals and is a zero-caloric natural alternative to artificial sweeteners. The direct organogenesis of&nbsp;<em>S.</em><em>rebaundiana</em>&nbsp;was carried out using apical bud explant&nbsp;<em>in</em><em>vitro</em>. The Murashige and Skoog medium was used and the culture was set up under optimized conditions of 24&plusmn;2&deg;C temperature, and 16 hours of light exposure with cool, white fluorescent light. The seven different concentrations of benzylaminopurine (BAP) and IAA (Indole-3-acetic acid) (0.01, 0.02, 0.03, 0.04, 0.05, 0.06 and 0.07mg/L) were employed for shoot development and root development respectively. The results revealed that of all the tested concentrations of BAP used for shoot development, 0.07mg/L proved to be best for shoot initiation after 6 days of culture. On the other hand, the suitable concentration of IAA at which root initiation was observed after 7 days of subculture, was 0.06mg/L. The study can be beneficial for the researchers, food industry and growers for commercial production of the&nbsp;<em>S.rebaudiana</em> nursery.</p> Direct organogenesis, Mass production, Medicinal plant, Steviarebaudiana, Tissue culture Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-623