17 Bellaouar Nacira Bouziane Mebarka Laichi Yacine Hadjadj Mohamed Hammoudi Roukia Hadj Mahammed Mahfoud Journal of Animal and Plant Sciences JAPS 20232023/08/04 33 4 971-977 1018-7081 https://doi.org/10.36899/JAPS.2023.4.0690 <p>The identification and characterization of two phenolic compounds from the ethyl acetate (EtAc) extract of&nbsp;<em>Calotropis procera&nbsp;</em>aerial parts were performed using chromatographic and spectroscopic methods namely Thin Layer Chromatography (TLC), Preparative Thin Layer Chromatography (PTLC), High-Performance Liquid Chromatography (HPLC), Infrared spectroscopy (FT-IR) and Nuclear Magnetic Resonance (¹H-NMR,&nbsp;¹³C-NMR).Two major compounds were isolated to be the 3, 3&rsquo;, 4&rsquo;, 5, 7-pentahydroxy flavones-3-rutinoside (Rutin) and 3,4,5-trihydroxybenzoic acid(Gallic acid), the results were confirmed by comparison with standards. In addition, the insecticidal activity of these isolated compounds was determined against&nbsp;<em>Parlatoria blanchardi</em><em>targ&nbsp;</em>individuals, 71.73% and 59.44% mortality were recorded at 0.5 mg/ml of Rutin and Gallic acid respectively, after 24 h of treatment; whereas, the larval stages were the most sensitive to Rutin treatment (LD_50&nbsp;equal to0.10 mg/ml for 1^st&nbsp;larva and 0.18 mg/ml for 2^nd larva).</p> Calotropis procera, Rutin, Gallic acid, Parlatoria blanchardi Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-612