17 Kwena Mokoena Thobela Louis Tyasi Vusi Mbazima Journal of Animal and Plant Sciences JAPS 20232023/09/30 33 5 1252-1257 1018-7081 https://doi.org/10.36899/JAPS.2023.5.0717 <p><span lang="EN-GB">Insulin-like growth factor 1 gene (<em>IGF-1</em>) is associated with growth traits in different livestock, but its association with growth traits in South African indigenous goat breeds is poorly understood. The study's objective was to identify the single nucleotide polymorphisms (SNPs) of&nbsp;<em>IGF-1</em>&nbsp;in the Kalahari Red goat breed and their&nbsp;</span>association with the growth traits. Two genotypes (KK and KM) were identified using polymerase chain reaction-restriction fragment length polymorphism (<span lang="EN-GB">PCR-RFLP). The frequency of genotype KK and KM was 0.56 and 0.44 respectively. The allelic frequency of allele K and M was 0.78 and 0.22 respectively. The studied Kalahari Red goat breed population was not in Hardy Weinberg equilibrium (HWE) as revealed by the Chi-square test (&chi;2 = 0.39). Marker-trait association indicated that the identified genotypes had no association (<em>P</em>&nbsp;&gt; 0.05) with the measured growth traits. In conclusion, the current study suggests that two identified genotypes of&nbsp;<em>IGF-1</em>&nbsp;might not be used as potential genetic markers during selection to improve growth traits. Further studies need to be conducted on SNPs of&nbsp;<em>IGF-1</em>&nbsp;and their association with growth traits using a larger sample, more growth traits and targeting more exons of</span><span lang="EN-GB">Kalahari Red goats.</span></p> Single nucleotide polymorphisms, Body weight, Genetic markers, Hardy Weinberg equilibrium Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-474