17 Noor Hashida Hashim Noor Eliza Hashim Faridah Sonsudin Noor Hazwani Hamidy Amirah Baharin Journal of Animal and Plant Sciences JAPS 20242024/01/20 34 1 157-167 1018-7081 https://doi.org/10.36899/JAPS.2024.1.0708 <p>Forty Sprague-dawley male rats were divided into 4 groups; Control, rats were forced feed with distilled water, 35 days; Morphine, rats were intramuscularly injected with morphine (20 mg/kg), 7 days;&nbsp;<em>P. dactylifera</em>&nbsp;(date), rats were forced feed with date&nbsp;<span lang="EN-MY">aqueous</span>&nbsp;extract, 28 days, and Morphine-<em>P. dactylifera</em>, rats were injected with morphine (20 mg/kg), the first 7 days and forced feed with date aqueous extract, another 28 days. The testis and epididymis were harvested for histological and sperm parameter analysis. The Morphine-&nbsp;<em>P. dactylifera</em>&nbsp;group showed significantly higher sperm count (109.30 &plusmn; 5.01 x 10⁶&nbsp;sperm/ml) and motility (72.80 &plusmn; 4.48 x 10⁶&nbsp;sperm/ml) than the morphine group (P &lt; 0.05). Significantly higher normal and lower abnormal sperm were observed in the Morphine-&nbsp;<em>P. dactylifera</em>&nbsp;group in comparison to the Morphine group. Higher in life sperm (90.50 &plusmn; 0.00 %) and lower in dead sperm (9.50 &plusmn; 0.00 %) were also observed in the Morphine-&nbsp;<em>P. dactylifera</em>&nbsp;group than the Morphine group. Testicular cells were found to be significantly higher in Morphine-&nbsp;<em>P. dactylifera</em>&nbsp;group as compared to the Morphine group. These research findings have provided additional information on the beneficial effect of&nbsp;<em>P. dactylifera</em>&nbsp;to ameliorate the damages caused by morphine on sperm parameters and testicular histoarchitecture. Thus, this study may suggest the potential use of&nbsp;<em>P. dactylifera</em> as a supplement to improve male reproductive functions.</p> Phoenix dactylifera, morphine, sperm, testis, rats Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-290