17 Xinxing DONG Ziyu LIU Xiaoyi WANG Qiang CHEN Gaicui BAI Guoxiang LAN Qian WANG Mingli LI Dawei YAN Shaoxiong LU Journal of Animal and Plant Sciences JAPS 20232023/12/15 33 6 1286-1291 1018-7081 https://doi.org/10.36899/JAPS.2023.6.0668 <p>Pituitary specific transcription factor-1 (<em>Pit</em>-1) gene is responsible for pituitary development and growth hormone expression, which is considered a pivotal candidate gene for growth in pig. This study aims to detect novel single nucleotide polymorphisms (SNPs) in porcine<em>&nbsp;Pit</em>-1 gene, investigate its effect on growth traits in the Duroc, Landrace and Yorkshire pigs. Two novel polymorphisms were detected, one located in the intron 2 (g. 13700 A&gt;G) and another located in exon 4 (g.25783 C&gt;T) which caused an amino acid change from threonine to methionine (Thr36Met). At g.13700 A&gt;G site, the frequency of A allele was higher than G allele in Duroc, Landrace and Yorkshire. At the g.25783 C&gt;T site, it was remarkable differences in allelic frequencies and genotypic frequencies among the three pig breeds. This study demonstrated the significant genotype effect of&nbsp;<em>Pit-1</em>&nbsp;on body weights at 70 d, age when reached 30kg, 50kg, 100kg and average daily gain from 30kg to 100kg. According to our results, the novel&nbsp;<em>Pit</em>-1 polymorphisms may be useful in pig selection as molecular markers and required in future studies.</p> Pit-1, pig , growth traits, polymorphisms Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-146