17 BUFFALO BULL SPERMATOZOA (BUBALUS BUBALIS) S. Iqbal A. Riaz S. Naz M. I. Rashid Syed M. H. Andrabi N. Ahmad Journal of Animal and Plant Sciences JAPS 20192019/12/01 29 6 1800-1802 1018-7081 NA <p>Present study was designed to ascertain antioxidative enzyme shift during cryopreservation of buffalo bull spermatozoa. Enzymatic profile of three enzymes (catalase, superoxide dismutase and glutathione peroxidase/reductase) was established for fresh, post-dilution, pre-freezing and post-thaw stages. A total of twenty semen samples from four bulls were cryopreserved in French straws by using tris based extender. Catalase (U/mL), superoxide dismutase (U/mL) and total glutathione (&micro;M) profile for fresh semen (25.8&plusmn;1.6, 93.1&plusmn;1.3, 77.4&plusmn;5.2; respectively), lowered with semen extension (6.0&plusmn;0.6, 60.7&plusmn;2.4, 63.0&plusmn;0.9 respectively; P&lt;0.05).During equilibration time, the enzymatic profile for catalase remained stable (7.0&plusmn;0.7; P&gt;0.05) but superoxide dismutase and glutathione profile deteriorated further (47.7&plusmn;1.4, 44.6&plusmn;0.6 respectively; P&lt;0.05). During cryopreservation, major shift was observed in catalase profile (2.3&plusmn;0.3; P&lt;0.05) while superoxide dismutase and glutathione readings were stable in post-thaw evaluations (P&gt;0.05). It is concluded that cryopreservation process deteriorated catalase enzyme profile while superoxide dismutase and glutathione profiles are declined by metabolic activities of spermatozoa in time dependent manner.</p> Buffalo semen, Cryopreservation, Antioxidant enzyme activity Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-633