17 S. Ali H. N. Hameed Journal of Animal and Plant Sciences JAPS 20192019/06/01 29 3 881-888 1018-7081 NA <p>Biological active secondary metabolite parietin was extracted from wild-type and mutant strain of lichen&nbsp;<em>Xanthoriaparietina</em>. Six different solvents (acetone, ethanol, methanol, hexane, chloroform and distilled water) were used to extract parietin. The parietin activity was found to be maximum when extraction was carried out with 3 ml of methanol at 30&ordm;C for 1h. The extracts were proceeded to antibacterial activity against different strains of bacteria.Anion DPPH (1,1-Diphenyl-2-picrylhydrazyl) scavenging, reducing power and total flavonoid content were used to analyze the antioxidant activity. HPLC analysis represented more than 90% purity of methanol extract with some trace elements. The antioxidant activity increased with increasing concentration of methanol extract. EMS treated strain represented maximum DPPH scavenging activity i.e., 84&plusmn;3.36% which was 2.08 fold higher than the wild-type. Maximum reduction potential was found to be 0.237&plusmn;0.0118 which was 1.83 fold higher than wild-type. Mutagen concentration of 0.5 mM exhibited strong parietin activity. The extracts of EMS treated mutant variant revealed strong antimicrobial activity (22 mm for bacteria) as compared to the wild-type and MMS treated variant. It was concluded that by increasing mutagen exposure time, the parietin activity decreased in terms of its antimicrobial activity.</p> Xanthoriaparietina, parietin, chemical mutagen, antioxidant, antimicrobial, secondary metabolite Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-331