17 M. M. Khatun P. K. Roy Md. Abdur Razzak Journal of Animal and Plant Sciences JAPS 20182018/04/01 28 2 589-596 1018-7081 NA <p>Plant needs some essential nutrient as well as growth regulator for their growth and development. In the case of Carnation (<em>Dianthus caryophyllus</em> L.) micro-propagation, large scale production was not possible by using the available traditional media (MS media) and method. In this study we first demonstrated the <em>in vitro</em> effect of coconut water (CW) together with cytokinine hormone on efficient growth of Carnation. The highest percentage of shoot induction was observed when the medium was used with coconut water (cw) at 10% +BAP 1.0 mg/l. In this case multiplication rate of carnation increased more than four times when shoot tip and nodal segment were used as an explant. So our modified media could be useful for Carnation (<em>Dianthus caryophyllus</em> L.) micro propagation and be a powerful media for tissue culture of Carnation. The best Carnation growth was observed in half-strength MS supplemented with 1.0 mg/l NAA for root induction. The survival rate of the transplanted plantlets was 80% in potted soil condition.</p> Micro propagation, Carnation, Media, Coconut water, Growth regulator Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-230