17 A. Rasool A. A. Anjum M. Rabbani M. Lateef F. Akhter H. Afroz J. Muhammad M. Nawaz Journal of Animal and Plant Sciences JAPS 20182018/04/01 28 2 491-497 1018-7081 NA <p><em>Mycoplasma synoviae</em>&nbsp;is one of the paramount causes of economic losses for poultry industry. Present study was carried out with the aim to isolate, identify and characterize&nbsp;<em>M. synoviae</em>&nbsp;from suspected chicken. Tracheal and cloacal swabs samples (n=500), collected from suspected chicken, were cultured on Modified Frey, s medium for isolation of&nbsp;<em>Mycoplasma</em>. Preliminary identification of&nbsp;<em>Mycoplasma</em>&nbsp;was done by culturing and biochemical characterization. Partial 16 S r RNA was amplified and sequenced for confirmation and molecular identification of&nbsp;<em>M. synoviae</em>. For strain identification and phylogenetic analysis, partial amplification and sequence of vlhA gene was performed. Out of 500 samples,&nbsp;<em>Mycoplasma&nbsp;</em>was successfully isolated from 40 samples. Out of 40 mycoplasma isolates, 20 were identified as&nbsp;<em>M. synoviae&nbsp;</em>by specie specific 16S ribosomal RNA gene, partial amplification (~213 bp). The vlhA gene was also successfully partially amplified (~375 bp) and sequenced from five isolates. GenBank accession numbers of reported local isolates in this study are KJ130523-KJ130542.and KP316017-KP316021 respectively.</p> Mycoplasma, fried egg colonies, 16S rRNA, vlhA gene Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-216