17 B. Tuncer Journal of Animal and Plant Sciences JAPS 20172017/02/01 27 1 277-282 1018-7081 N/A <p>In this study, the effects of applications of colchicine on microspore cultures were evaluated in 4 radish (<em>Raphanussativus</em> L.) varieties. For this purpose, 5 different concentrations of colchicine (0, 10, 25, 50, and 75 mg L-1) were applied under dark conditions at 4&deg;C to microspores that had been isolated in Gamborg&rsquo;s medium containing 13% (w/v)sucrose (B5-13). After the application of colchicine the microspores were kept for 1 day at 32.5&deg;C in1/2 x Nitsch and Nitschliquid media supplemented with13% (w/v)sucrose (1/2 NLN-13) at a density of 40.000 microspore mL-1and transferred for culturing under dark conditions at 25&deg;C. Colonies of callus were observed in Petri dishes 4&ndash;5 weeks after the microspore isolation. In this study, the 50 mg L-1 dose of colchicine was found to be more effective in terms of callus formation. The highest callus formation was achieved from the large round radish (cv. Burkır) (6.63 calli/Petri dish)and the standard small rooted radish (cv. Cherry Belle)(5.73 calli/Petri dish) varieties.</p> Microspore culture, callus, colchicine, Raphanussativus L Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-34