17 Y. Liu P. Lib L. Zhao Z. Zhao Y. Li M. Liu X. Yang L. Zan Journal of Animal and Plant Sciences JAPS 20172017/12/01 27 6 1847-1851 1018-7081 NA <p>The liver X receptor &alpha; (<em>LXR&alpha;)&nbsp;</em>is a member of the nuclear hormone receptor superfamily which could regulate the transcription of the genes involved in cholesterol transportation. In this study, Lentiviral vector containing<em>&nbsp;LXR&alpha;&nbsp;</em>shRNAs were constructed and transferred in bovine muscle satellite cells. The results indicated that&nbsp;<em>LXR&alpha;&nbsp;</em>down regulated&nbsp;<em>PPAR&alpha;</em>,&nbsp;<em>PPAR&gamma;</em>,&nbsp;<em>ABCA1</em>,&nbsp;<em>LPL</em>,&nbsp;<em>ApoE&nbsp;</em>genes after 48 h post-pLenti-03 viruses' infection. The silencing effects of bovine&nbsp;<em>LXR&alpha;&nbsp;</em>gene on related were significant. This work identifies bovine<em>&nbsp;LXR&alpha;&nbsp;</em>role in cholesterol metabolism.</p> bovine LXRα gene, lentivirus, vector construction, gene silencing Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-234