17 A. Etminan A. Pour-Aboughadareh R. Mohammadi A. Ahmadi-Rad Z. Moradi Z. Mahdavian A. Noori Journal of Animal and Plant Sciences JAPS 20172017/10/01 27 5 1582-1587 1018-7081 NA <p>Durum wheat is important cereal that is widely cultivated all over the world. In this study, genetic diversity in a mini core collection of the 43 durum wheat genotypes (including 25 breeding lines and 18 landraces) was investigated using fifteen inter-simple sequence repeat markers (ISSR). Based on the results, high levels of polymorphism (98.70%) were observed, which indicated that ISSR was efficient technique to detect the genetic variation of this collection. Analysis of molecular variance (AMOVA) revealed high genetic variability (90%) within breeding lines and landrace genotypes. Comparing the genetic diversity of breeding lines and landraces, based on genetic variation parameters, revealed that the effective number of alleles (<em>Ne</em>), Nei's gene diversity (<em>H</em>) and Shannon's information index (<em>I</em>) in the landrace genotypes is higher than breeding lines. Cluster analysis classified all individuals in five groups. Also, inter-population differentiation (<em>Gst</em>) estimated on the basis of these primers represented that, 93% of the total genetic variability refer to variation within two sets of genotypes. Overall, our results revealed a high level of genetic variation among a mini core collection of durum wheat, especially Iranian landraces, which can be of interest for conservation and future breeding programs.</p> Durum wheat; genetic variation; landrace genotypes; ISSR marker Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-200