17 R. Y. Wang H. G. Wang X. Y. Liu S. Lian L. Chen Z. J. Qiao C. E. McInerney L. Wang Journal of Animal and Plant Sciences JAPS 20172017/08/01 27 4 1303-1314 1018-7081 NA <p>As a well-adapted plant to dry soil and short growing seasons, common millet holds great potential as a drought-resistant crop. Here, we performed a comparative RNA-Seq analysis of two common millet genotypes, Huangmizi (tolerant) and Zhenyuandamizi (sensitive) grown under stress conditions (20% Polyethylene Glycol 6000 with an average molecular weight of ～5,400-7,000 solution). Leaves of seedling plants were harvested from a control and also after 1 and 3 h stress treatment and pooled for RNA isolation. Using Illumina paired-end sequencing, approximately 46.5 million clean reads were generated. Following a<em>&nbsp;de novo</em> assembly, a total of 42,240 Unigenes were obtained. A total of 2,301 SSRs and 1,447,148 SNPs were also identified and 75% of Unigenes were annotated. A total of 21,556 and 8,304 Unigenes aligned to the GO and COG databases, respectively. The GO classification showed that the Unigenes were distributed into three categories with 74.30% having biological functions, 76.51% having molecular functions and 79.30% having cellular-level functions. By cross-referencing against KEGGs, 5,535 Unigenes were assigned to 63 metabolic pathways. Additionally, 701 DEGs including 187 up-regulated and 514 down-regulated genes were detected. Transcripts identified in this study will accelerate our understanding of the molecular mechanisms of drought tolerance in common millet.</p> Common millet (Panicum miliaceum L.), Drought, Transcription Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-165