17 B. Niaz1 M. I. Rajoka K. A. Al-Ghanim S. Yousaf S. Mahboob S. Nadeem Journal of Animal and Plant Sciences JAPS 20172017/08/01 27 4 1217-1224 1018-7081 NA <p>&nbsp;Various concentrations of biotin were tested in order to achieve fluent excretion of glutamic acid (glu) in a fermentation medium composed of a locally isolated&nbsp;<em>Corynebacterium glutamicum</em>&nbsp;strain. The behaviour of the strain was observed to vary with varying concentrations of biotin. Maximum values for specific growth rate (&mu;, h-1), cell mass yield (Yx/s, g/g), substrate consumption rate (QS, g/l h) and cell mass specific productivity (qX, g/g h) were 0.34, 0.24, 2.92 and 1.43, respectively, given 10 &mu;g biotin/100 ml under working conditions of a shake flask with temperature 30 &deg;C, in 100 g glucose/l medium (pH 7.0). The kinetic parameters calculated for glutamate production under the above conditions were 4.94 g/l h, 4.4 g/g cells, 0.66 g/g, and 1.5 g/g .h for QP, YP/X, YP/S, and qP, respectively, and were comparatively higher than the respective values reported for some&nbsp;<em>C. glutamicum</em>&nbsp;strains growing on glucose in batch culture studies. Conversely, at biotin concentrations of 1.0, 2.5, 5.0, 15 &mu;g/ ml, significantly less improvement in&nbsp;<em>glu</em> production was noticed, but at 10 &mu;g/ml a nearly 2.2-fold increase in QP was observed, though the only statistically significant difference was in the QS value.</p> Biotin; optimization; glutamic acid Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-154