17 F. Ambreen M. Javed Journal of Animal and Plant Sciences JAPS 20162016/06/01 26 3 674-679 1018-7081 NA <p>The present study was undertaken to examine the DNA damage induced by binary metal mixture (cadmium-lead) in peripheral blood erythrocytes of freshwater carp,&nbsp;<em>Cyprinus carpio</em>&nbsp;by using Comet bioassay. The 96-hr LC50 value of mixture was estimated for 180-old fingerlings of&nbsp;<em>Cyprinus carpio&nbsp;</em>in a static system and then four sub-lethal concentrations viz. SL-I (10.42mgL-1), SL-II (7.81mgL-1), SL-III (6.25mgL-1) and SL-IV (5.21mgL-1) were calculated and fishwere exposed to these concentrations, separately in glass aquaria for 30 days along with negative and positive control at constant water temperature (30&ordm;C), pH (7.75) and total hardness (225mgL-1). Peripheral erythrocytes were sampled after 30 day exposure for DNA damage assessment. Statistically significant effects were observed at sub-lethal concentrations in-terms of percentage of DNA damage, cumulative tail lengths (&micro;m) and genetic damage index. Concentration dependent response was observed in fish erythrocytes with induction of maximum DNA damage, due to positive control, followed by at highest concentration (SL-I) of mixture. This study also concluded that comet bioassay can be used for in-vivo experiments, using fish as a model for the screening of genotoxic and mutagenic pollutants in aquatic environment.</p> Carp, Comet bioassay, DNA damage, Metal Mixture, Sub-lethal Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-92