17 H. Xiao Z. Zhao X. Fang H. Yu X. Long P. Jiang R. Yang Journal of Animal and Plant Sciences JAPS 20162016/06/01 26 3 658-664 1018-7081 NA <p>Acyl coenzyme A long-chain 5 synthetase (ACSL5), a member of the ACSL family, plays a key role in fatty acid beta-oxidation and fat synthesis in mammals.<a id="OLE_LINK25" name="OLE_LINK25"></a>&nbsp;In this study,&nbsp;two SNPs&nbsp;<a id="OLE_LINK15" name="OLE_LINK15"></a><a id="OLE_LINK14" name="OLE_LINK14"></a>g.33185918G&gt;A&nbsp;and&nbsp;<a id="OLE_LINK21" name="OLE_LINK21"></a><a id="OLE_LINK20" name="OLE_LINK20"></a>g.33186348C&gt;T&nbsp;in the 5&rsquo; flanking sequence of the bovine ACSL5 gene were identified by PCR-RFLP. Thus, we aimed to evaluate the association between the two SNPs and meat quality traits, in addition to the carcass quality of Chinese Simmental-cross steers. The two SNPs were genotyped, and 36 carcass and meat quality traits were measured in a population of 354&nbsp;<a id="OLE_LINK45" name="OLE_LINK45"></a>individuals. Statistical analysis revealed that the two SNPs were significantly associated with carcass and meat quality traits. g.33185918G&gt;A was very significantly (p&lt;0.01) associated with the weight of the omasum and the pH of beef. For g.33186348C&gt;T, the weight of the liver, hind hooves, kidney, and testis and the fat coverage rate of the carcass were very significantly associated (p&lt;0.01). The present work indicated that polymorphisms in ACSL5 might be important genetic factors affecting meat quality and carcass yield in Chinese Simmental-cross steers. Moreover, such polymorphisms may serve as practical markers for carcass and meat quality traits in future marker-assisted selection (MAS) programs in beef cattle breeding and production.</p> ACSL5, Chinese Simmental cattle, SNPs, Meat quality trait, MAS Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-90