17 P. Sofia M. Asgher M. Shahid M. A. Randhawa Journal of Animal and Plant Sciences JAPS 20162016/10/01 26 5 1451-1463 1018-7081 NA <p>The lignin mineralizing enzymes system of white rot fungi has tremendous catalytic prospect for oxidative bioremediation of a number of toxic pollutants and many other industrial uses. Immobilization enables the reuse of enzymes and making them industrially relevant and economical biocatalysts.&nbsp;<em>Schizophyllum commune&nbsp;</em>IBL-06 was cultivated for the secretion of lignin peroxidase (LiP) in pre-optimized solid state fermentation medium of corn stover. A high yield of lignin peroxidase (1347.3 U/mL) was recorded in crude culture supernatant. LiP was purified (5.65 folds) by a pre-standardized four step protocol comprising ammonium sulphate fractionation, dialysis, DEAE cellulose ion exchange and Sephadex G-100 column chromatography. The 38 kDa single polypeptide&nbsp;<em>S. commune&nbsp;</em>IBL-06 LiP migrating as a single clear band on both native and SDS-PAGE gels. The purified enzyme was then immobilized on chitosan beads activated with gluteraldehyde (cross-linker).Scanning electron microscopy (SEM) was conducted for the confirmation of LiP attachment on chitosan beads. The highest textile dye decolorization (95.45%) potential was observed with chitosan-immobilized enzyme at 30&deg;C without hemolytic toxicity. The chitosan beads-LiP retained approximately more than 70% activity after three repeated runs that gradually decreased to 35 % after 7th cycle of reusability. The immobilized LiP was found to show superior dye removal properties as compared to free LiP. Higher thermo stability, lower K<em>m</em>and high V<em>max&nbsp;</em>features of chitosan beads immobilizedLiP suggested its suitibility for variousbiotechnological and industrial applictaions.</p> Schizophyllum commune IBL-06, lignin peroxidase, chitosan beads immobilization, characterization, dye decolorization Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-191