17 E. Mohamed F. A. Rahiman R. A. Wahab C. R. C M. Zain M. A Javed F. Huyop Journal of Animal and Plant Sciences JAPS 20162016/08/01 26 4 1133-1139 1018-7081 NA <p><a id="_GoBack" name="_GoBack"></a>A<em>&nbsp;dehalogenase D</em>&nbsp;gene (<em>dehD</em>) capable of degrading monochloroacetic acid (MCA) has been previously isolated from&nbsp;<em>Rhizobium</em>&nbsp;sp. RC1 and characterized. The 804-bp<em>&nbsp;dehD</em>&nbsp;gene was inserted into pCAMBIA under the control of the cauliflower mosaic virus 35S promoter and designated pCAMdehD, with a total size of 10,592 bp. The pCAMdehD was introduced into tobacco (<em>Nicotiana benthamiana</em>) via&nbsp;<em>Agrobacterium</em>-mediated transformation. The integration and expression of&nbsp;<em>dehD</em>&nbsp;in&nbsp;<em>N. benthamiana&nbsp;</em>was confirmed by PCR and reverse transcription PCR, respectively. MCA-resistant transformants were selected in tissue cultures containing 60 &micro;g/L MCA. Analysis of plants using a leaf-painting assay revealed that transgenic&nbsp;<em>N. benthamiana&nbsp;</em>was resistant to4.0 g/L MCAcompared with 2.0 g/L for non-transformed controls. The use of&nbsp;<em>dehD</em>&nbsp;could thus be advantageous for herbicide-resistant plant breeding systems, and it is also a suitable marker gene for plant-transformation studies. To the best of our knowledge, this is the first report detailing transgenic&nbsp;<em>N. benthamiana</em>&nbsp;engineered with&nbsp;<em>dehD</em>&nbsp;from&nbsp;<em>Rhizobium&nbsp;</em>sp. RC1.</p> Binary vector, dehD gene, Herbicide resistant, Monochloroacetic acid, Tobacco Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-148