17 F. Wadood M. Aleem A. Ijaz N. Ahmad M. S. Yousaf D. H. Mughal A. Javid 8and S. A. Mahmood Journal of Animal and Plant Sciences JAPS 20152015/02/01 25 1 301-303 1018-7081 NA <p>This study was designed to determine effect of various concentrations of L- cysteine on frozen-thaw semen quality of Nili Ravi buffalo. Single ejaculate of four buffalo bulls (Nili-Ravi) was collected at weekly intervals for 5 weeks. Ejaculates of acceptable motility (&ge;70%) were pooled and semen was diluted by tris citric acid extender (TCAE; 300 mosm/kg osmotic pressure and ~7.0 pH) having 4 levels of L-cysteine (0.0, 2.0, 5.0 and 8.0 mM). Semen was equilibrated at 4˚C and packaged in 0.5 mL French straws with 20&times;106 spermatozoa/ straw and then frozen in liquid nitrogen. L-cysteine inclusion levels had non-significant (P&gt; 0.05) effect on spermatozoa motility, viability, NAR, PMI and on lipid peroxidation. However, DNA damage in spermatozoa was significantly higher (P&lt;0.05) in 8.0 mM L-cysteine. It was concluded that L-cysteine (2.0 and 5.0 mM) addition to cryopreserved semen had no significant effect on post thaw semen characteristics, except at 8 mM which adversely affected DNA integrity. This may be due to its toxicity at this concentration.</p> cryopreservation, semen, spermatozoa, antioxidants, buffalo, L-cysteine Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-42