MOLECULAR CHARACTERIZATION OF THE σC-ENCODING GENE FROM NOVEL DUCK REOVIRUS

T. Yun H. P. Chen J. G. Hua B. Yu L. Chen Z. Ni W. C. Ye C. Zhang MOLECULAR CHARACTERIZATION OF THE σC-ENCODING GENE FROM NOVEL DUCK REOVIRUS

Journal of Animal and Plant Sciences

JAPS

2015

2015/06/01

25 3 33-41 1018-7081

<p>We cloned and sequenced the σC-encoding gene of a novel duck reovirus (N-DRV) isolate ZJ00M. The σC gene is 966 bp long and is encoded by the third ORF of the S1 segment, a small protein of 321 amino acid residues (approximately 34.1 kDa). Expressed σC fusion protein in E. coli showed good antigenicity and immunogenicity in a western blotting assay and indirect immunofluorescence assays (IFA). The comparative sequence analyses indicated that N-DRV σC shared high identity levels with ARV species (classical MDRV and ARV) and a lower degree of identity with other orthoreovirus counterparts, but the sequence identities were no more than 45 %. As observed in classical MDRV and ARV σC protein sequences, some sequence motifs, conserved sequence motifs and/or functional domains were identified in the sequence of N-DRV σC. The phylogenetic analysis of σC/σ1 sequence revealed that N-DRV was different fromother orthoreovirus species groups but remained classified into the avian orthoreoviruses along with other ARVs, suggesting that N-DRV and classical MDRVs constitute a separate geno group relative to ARV. These results are important for future studies examining the structure, function and immunogenicity of N-DRV σC.</p> Novel duck reovirus, σC-encoding gene, Expression, Phylogenetic analysis, Genotype Pakistan Agricultural Scientists Forum

https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-306